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11388908910

Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

Synonyme(s) :

Biotin-16-UTP, biotin

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About This Item

Code UNSPSC :
41116100

Pureté

97.7% (HPLC)

Niveau de qualité

100

Forme

solution

Poids mol.

Mr 987.5 (biotin-16-UTP-Li4)

Conditionnement

pkg of 25 μL (250 nmol; 10mM)

Fabricant/nom de marque

Roche

Couleur

colorless

Solubilité

water: miscible

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Qualité

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Forme physique

Biotin-16-UTP, tetralithium salt, 10mM solution

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Damian LaGamba et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 234(1), 132-142 (2005-07-13)
One of the most fundamental biological processes in development, as well as a primary mechanism for tumor metastasis, is epithelial-mesenchymal transformation (EMT). To gain a greater understanding of this transition, we have obtained a genomic profile of the critical stages
Chenying Li et al.
Haematologica, 105(1), 148-160 (2019-04-13)
Homoharringtonine, a plant alkaloid, has been reported to suppress protein synthesis and has been approved by the US Food and Drug Administration for the treatment of chronic myeloid leukemia. Here we show that in acute myeloid leukemia (AML), homoharringtonine potently
Subramaniam Jayanthi et al.
PloS one, 4(6), e6092-e6092 (2009-07-01)
Methamphetamine (METH) is an illicit toxic psychostimulant which is widely abused. Its toxic effects depend on the release of excessive levels of dopamine (DA) that activates striatal DA receptors. Inhibition of DA-mediated neurotransmission by the DA D1 receptor antagonist, SCH23390
Aikaterini Tsaousi et al.
Circulation research, 108(4), 427-436 (2011-01-05)
Vascular smooth muscle cell (VSMC) proliferation causes intimal thickening in atherosclerosis and restenosis. Previously, we demonstrated that Wnt/β-catenin signaling upregulates VSMC proliferation in vitro. We examined this pathway in vivo and investigated the involvement of specific Wnt proteins in VSMC

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