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11383213001

Roche

NBT

4-Nitro blue tetrazolium chloride, solution

Synonyme(s) :

Nitroblue tetrazolium chloride

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About This Item

Code UNSPSC :
41116100

Niveau de qualité

100

Pureté

>95%

Forme

solution

Poids mol.

Mr 817.7

Conditionnement

pkg of 3 mL (300 mg)

Fabricant/nom de marque

Roche

Technique(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

Température de stockage

−20°C

Description générale

NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action.

Application

  • Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Forme physique

Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.

Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.

Notes préparatoires

Working solution: Preparation of staining solution
  • Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
  • Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Exclamation markHealth hazard
GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H319,H332,H360D

Classification des risques

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Repr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

167.0 °F

Point d'éclair (°C)

75 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Stefan Dhein
Practical Methods in Cardiovascular Research (2006)
Ewa Pocheć et al.
Acta biochimica Polonica, 62(2), 323-328 (2015-06-23)
Although both uveal (UM) and cutaneous (CM) melanoma cells derive from the transformed melanocytes, their biology varies significantly in several aspects. Malignant transformation is frequently associated with alternations in cell glycosylation, in particular those concerning branched complex-type N-glycans. These changes
J Omar Yáñez-Cuna et al.
Genome research, 22(10), 2018-2030 (2012-04-27)
The regulation of gene expression is mediated at the transcriptional level by enhancer regions that are bound by sequence-specific transcription factors (TFs). Recent studies have shown that the in vivo binding sites of single TFs differ between developmental or cellular
Andrew R Bassett et al.
Nature communications, 5, 4640-4640 (2014-08-20)
MicroRNA (miRNA) target recognition is largely dictated by short 'seed' sequences, and single miRNAs therefore have the potential to regulate a large number of genes. Understanding the contribution of specific miRNA-target interactions to the regulation of biological processes in vivo
Zoe Crane-Smith et al.
Frontiers in cell and developmental biology, 9, 595744-595744 (2021-04-20)
Enhancers that are conserved deep in evolutionary time regulate characteristics held in common across taxonomic classes. Here, deletion of the highly conserved Shh enhancer SBE2 (Shh brain enhancer 2) in mouse markedly reduced Shh expression within the embryonic brain specifically
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Protocoles

NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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