10102105001
Roche
Adenosine Deaminase (ADA)
from calf intestine
Synonyme(s) :
ADA
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About This Item
Produits recommandés
Source biologique
bovine (calf) intestine
Niveau de qualité
Forme
solution
Activité spécifique
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
Poids mol.
230-440 kDa (high)
30-47 kDa (low )
Conditionnement
pkg of 2 mL (10 mg)
Fabricant/nom de marque
Roche
pH optimal
7.0-7.4
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Catégories apparentées
Description générale
Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Application
Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.
Actions biochimiques/physiologiques
Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Qualité
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Forme physique
Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6
Notes préparatoires
Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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Regulation of MMP-9 expression by the A2b adenosine receptor and its dependency on TNF-alpha signaling
Chen H, et al.
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Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
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Activation of the macrophage A2b adenosine receptor regulates tumor necrosis factor-alpha levels following vascular injury
Chen H, et al.
Experimental Hematology, 37(5), 533-538 (2009)
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