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SCM302

Sigma-Aldrich

Definitive Endoderm Induction Medium

Synonyme(s) :

Definitive Endoderm Medium, Endoderm Induction Medium, Medium for Endoderm Induction

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.71

Forme

liquid

Conditionnement

pkg of 50 mL

Technique(s)

cell culture | stem cell: suitable

Impuretés

<2 EU/mL endotoxin

pH

7.0-7.4

Application(s)

cell analysis

Catégories apparentées

Description générale

The definitive endoderm generates the epithelial lining of the respiratory and digestive tracts as well as cell of the thyroid, thymus, lungs, liver and pancreas. The definitive endoderm induction medium (SCM302) is a defined, animal component-free, ready-to-use serum-free medium for efficient differentiation of human pluripotent stem cells (ES/iPSCs) to definitive endoderm (DE) cells.

The 3-day differentiation protocol is simple and has been validated on multiple human iPS cell lines. Differentiated cells express high levels (>85%) of endoderm markers including CXCR4, c-Kit, Sox-17 and FOXA2 and can be further differentiated into specialized endodermal cell lineages. Definitive Endoderm Induction Medium can be used to generate 3D organoids such as hiPS derived colon (Cat. No. SCC300) and lung organoids.

Application

Ready-to-use serum-free media for the efficient differentiation of human pluripotent stem cells (ES/iPSCs) to CXCR4+/c-Kit+/Sox-17+/FOXA2+ definitive endoderm (DE) cells.

Research Category:
Stem Cell Research

Qualité

Appearance: Clear liquid/no particulates
Sterility Tested: No growth
Endotoxin: <2 EU/mL
pH: 7.0-7.4
Mycoplasma: Negative
Functional Assay: >85% CXCR4+ c-Kit+ double positive after 3 days of induction.

Stockage et stabilité

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Articles

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Protocoles

In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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