S4030-M
Dextran Sulfate 50% Solution
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About This Item
Code UNSPSC :
41116012
eCl@ss :
32160405
Produits recommandés
Conditionnement
pkg of 100 mL
Niveau de qualité
Fabricant/nom de marque
Chemicon®
Technique(s)
hybridization: suitable (nucleic acid)
Description générale
Dextran Sulfate (MW >500,000) is used as an additive in probe hybridization solutions. Its presence accelerates the rate of hybridization by 10 fold. The addition of Dextran Sulfate favors the formation of probe networks, resulting in greater sensitivity during membrane-bound nucleic acid analysis. The product is supplied as a 50% (w/v) solution.
Application
Dextran Sulfate - 50% Solution
To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.
See the "HYBRISOL® Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.
To prepare a solution containing 10% Dextran Sulfate, add 8 ml of hybridization ingredients to 2 ml of 50% Dextran Sulfate Solution.
See the "HYBRISOL® Nomograph for Dextran Sulfate" link located under the "Protocols" tab at catalog number S4030 product specific page on www.chemicon.com for determining the proper concentration of dextran sulfate needed for various hybridization condition guidelines.
Research Category
All
All
Forme physique
A 50% dextran sulfate solution in water filtered through a 20 micron membrane and autoclaved prior to bottling in clean, sterilized bottles containing no preservatives. Liquid solution is dark brown from autoclaving.
Stockage et stabilité
Store at room temperature tightly sealed when not in use. Solution can be stored refrigerated however it should be warmed to at least room temperature prior to use dilution for best results.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
HYBRISOL is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
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