NE1013

Anti-PGP9.5 (176-191) Rabbit pAb

liquid, Calbiochem^®

• Synonyme(s) : Anti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Espèces réactives: porcine, human, rat, mouse
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UCHL1(7345)

Description

Description générale

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.

Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.

This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).

Immunogène

a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9

Application

Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
Mouse dorsal root nerve fibers

Autres remarques

Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.

PGP9.5 is also overexpressed in some cancers.



Immunofluorescence protocol

Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).

1. Warm slides to room temperature.

2. Re-hydrate with PBS for 10-15 min.

3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton^™ X-100 detergent, and 0.01% NaN₃, pH 7.45) for 1 h at room temperature.

4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.

5. Remove blocking buffer and add diluted primary antibody.

6. Incubate with primary antibody at 4°C for 18-48 h.

7. Remove primary antibody and wash three times for 10 min with PBS.

8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.

9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.

10. Remove secondary antibody and wash three times for 10 min with PBS.

11. Coverslip slides with appropriate mounting media.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1