MABT1346

Anti-P-Cadherin Antibody, clone CSTEM29

• Synonyme(s) : Cadherin-3;Placental Cadherin
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: CSTEM29, monoclonal
• Poids mol.: calculated mol wt 91.42 kDa; observed mol wt ~80 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): flow cytometry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_001304124
• Numéro d'accès UniProt: P22223
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CHD3(1001)

Description

Description générale

Cadherin-3 (UniProt: P22223; also known as Placental cadherin, P-cadherin) is encoded by the CHD3 (also known as CDHP) gene (Gene ID: 1001) in human. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in connecting cells. P-cadherin is a class I cadherin that is expressed in myoepithelial cells and is also expressed during late pregnancy and lactation, when luminal epithelial cells secrete high levels of a soluble fragment of P-cadherin in human milk. It is a single pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24) and a propeptide (aa 25-107 that are subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 108-654), a transmembrane domain (aa 655-677), and a cytoplasmic domain (aa 678-829). It has 5 cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections and thereby imparting a strong curvature to the full-length ectodomain. P-cadherin dysfunction is strongly associated with tumorigenesis and confers the malignant phenotype in cancer cells. Its overexpression is strongly associated with poor prognosis in several solid tumors. Its high expression is reported to be predictive of shorter overall survival in glioblastoma patients. It is also detected in plasma membrane during progression of pancreatic intraepithelial ductal adenocarcinoma. (Ref.: Martins, EP., et al. (2021). Mol. Oncol. doi: 10.1002/1878-0261; Yu, W., et al. (2019). Front. Oncol. 9; 989; Siret, C., et al. (2018). Br. J. Cancer. 118; 546-557).

Spécificité

Clone CSTEM3 is a mouse monoclonal antibody that detects P-Cadherin (Cadherin 3). It targets an epitope within the extracellular domain.

Immunogène

His-tagged recombinant protein fragment corresponding to the extracellular domain of human P-Cadherin (Cadherin 3).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant human P-Cadherin.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human P-Cadherin.

Tested Applications

ELISA Analysis: A representative lot detected P-Cadherin in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Flow Cytometry Analysis: A representative lot detected P-Cadherin in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected P-Cadherin in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-P-Cadherin, clone CSTEM29, Cat. No. MABT1346, is a mouse monoclonal antibody that detects P-Cadherin and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable