MABS1291

Anti-Cas9 Antibody, C-term. clone 10C11-A12

clone 10C11-A12, from mouse

• Synonyme(s) : CRISPR-associated endonuclease Cas9/Csn1, SpyCas9
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 10C11-A12, monoclonal
• Espèces réactives: bacteria
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_269215
• Numéro d'accès UniProt: Q99ZW2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. ′ Les clusters CRISPR sont transcrits et transformés en ARN CRISPR (ARNcr). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. ′′

Spécificité

Clone 10C11-A12 recognizes an epitope located in the C-terminal region of Cas9.

Immunogène

His-tagged recombinant S.pyogenes Cas9 C-terminal fragment.

Application

Analyse par immunocytochimie : µ
µµ
µµ
Clone 10C11-A12 hybridoma culture supernatant detected doxycycline treatment-induced expression of a FLAG-tagged Cas9 construct under the control of the PTight promoter in a HeLa-derived Tet-ON cell line (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).

Anti-Cas9 Antibody, C-term. clone 10C11-A12 is an antibody against Cas9 for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Qualité

µµ

Description de la cible

Poids réel (observé) : env. 158 kDa. 158,4 kDa (poids déterminé par calcul).

Forme physique

Format : Produit purifié

Autres remarques

Concentration : Voir la fiche technique du lot concerné.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable