MABN1529

Anti-PRICKLE2 Antibody, clone 3B4.1

clone 3B4.1, from mouse

• Synonyme(s) : Prickle-like protein 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 3B4.1, monoclonal
• Espèces réactives: human, mouse
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_942559
• Numéro d'accès UniProt: Q7Z3G6
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PRICKLE2(166336)

Description

Description générale

Prickle-like protein 2 (UniProt Q7Z3G6) is encoded by the PRICKLE2 (also known as EPM5) gene (Gene ID 166336) in human. Prickle2 is a nuclear membrane associated protein that belongs to a highly conserved Prickle, Espinas, Testin (PET) and Linl-1, Isl-1, and Mec-3 (LIM) domain-containing protein family. Prickle is part of the non-canonical Wnt signaling pathway that establishes planar cell polarity. It plays a critical role in neuron architecture and function. In mice it is shown to be highly expressed in the hippocampus region where it localizes to the post-synaptic density (PSD). It interacts the PSD-95 and NMDA receptor, the two proteins implicated in Autism spectrum disorders. Loss of Prickle2 is known to reduce number and size of miniature synaptic currents, indicating deficits in basal synaptic transmission. Mice deficient in Prickle2 exhibit lower seizure threshold and PRICKLE2 mutations in humans are associated with progressive myoclonic epilepsy type 5. Ref.: Sowers, LP., et al.. (2013), Mol. Psychiatry. 18, 1077-1089.

Spécificité

Clone 3B4.1 targets an internal PRICKLE2 epitope conserved between human and murine species.

Immunogène

GST-tagged recombinant human PRICKLE2 internal fragment.

Application

Anti-PRICKLE2 Antibody, clone 3B4.1, Cat. No. MABN1529, is a highly specific mouse monoclonal antibody that targets PRICKLE2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PRICKLE2 in mouse cerebral cortex, as well as in human pancreas, small intestine, and tonsil tissue sections.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PRICKLE2 in 10 µg of mouse brain tissue lysate.

Description de la cible

~95 kDa observed. 95.62/95.30 kDa (human pro-/mature form) and 95.78/95.47 kDa (mouse pro-/mature form) calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1