MABF851

Anti-ERAP1 Antibody, clone 16A7.1

clone 16A7.1, from mouse

• Synonyme(s) : Endoplasmic reticulum aminopeptidase 1, A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase,
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 16A7.1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001035548
• Numéro d'accès UniProt: Q9NZ08
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ERAP1(51752)

Description

Description générale

Endoplasmic reticulum aminopeptidase 1 (UniProt Q9NZ08; also known as A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator) is encoded by the ERAP1 (also known as ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILSAP) gene (ORF UNQ584/PRO1154; Gene ID 51752) in human. Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a single transmembrane protein belonging to the M1 family of zinc metallopeptidases. ERAP1 consists of an N-terminal transmembrane segment (a.a. 2-21) and a large lumenal domain (a.a. 22-941), with only the N-terminus methionine exposed cytoplasmically. ERAP1 processes peptides for major histocompatibility complex (MHC) class I presentation and promotes ectodomain shedding of several cytokine receptors, including TNF-R1, IL-6R alpha chain, and IL-1 type II decoy receptor (IL-1RII). Ankylosing spondylitis (AS) is a common, highly heritable form of inflammatory arthritis. Several ERAP1 single nucleotide polymorphic (SNP) variants are reported to influence the risk of AS in individuals carrying class I MHC allele HLA-B*27 and HLA-B*40:01.

Spécificité

Expected to react with both spliced isoforms of human ERAP1 (UniProt Q9NZ08-1 & Q9NZ08-2)

Immunogène

Epitope: Internal.

GST-tagged recombinant human ERAP1 internal fragment.

Application

Detect ERAP1 using this Anti-ERAP1 Antibody, clone 16A7.1 validated for use in Western Blotting.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected ERAP1 in 10 µg of K562 cell lysate.

Qualité

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ERAP1 in 10 µg of A431 cell lysate.

Description de la cible

~110 kDa observed. 107.2 kDa (isoform 1) and 107.8 kDa (Isoform 2) calculated.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable