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MABF3151

Sigma-Aldrich

Anti-Rabies Virus Phosphoprotein Antibody, clone M953

Synonyme(s) :

Protein P;Protein M1

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About This Item

Code UNSPSC :
12352203

Source biologique

mouse

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

M953, monoclonal

Poids mol.

calculated mol wt 33.21 kDa
observed mol wt ~44 kDa

Produit purifié par

using protein G

Espèces réactives

rabies virus

Conditionnement

antibody small pack of 100 μg

Technique(s)

ELISA: suitable
immunofluorescence: suitable
inhibition assay: suitable
western blot: suitable

Isotype

IgG1κ

Séquence de l'épitope

N-terminal half

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Catégories apparentées

Description générale

Phosphoprotein (UniProt: P69480; also known as Protein P, Protein M1) is encoded by the P gene in Rabies virus. Rabies virus belongs to a family of RNA viruses called Rhabdoviridae, genus Lyssavirus. It is most commonly transmitted to humans through the saliva of an infected animal. This genus of virus shares many of the biological and physicochemical features that are associated with other rhabdoviruses. They have similar mechanisms to enter susceptible cells, express and replicate their genome RNA and release mature virus particles from the plasma membrane of infected cells. Rabies virus prototype is organized into five coding regions, N, P, M, G, and L. Viral transcription and replication are catalyzed by the viral polymerase complex, composed of the L and P proteins. Rabies virus is reported to bind to nicotinic acetylcholine receptors at the neuromuscular junction and travels toward the spinal cord within axons of peripheral nerves by retrograde fast axonal transport. The virus disseminates within axons in the CNS along neuroanatomical pathways. It replicates in neurons and causes neuronal dysfunction. The phosphoprotein of Rabies virus is a non-catalytic polymerase D12cofactor and regulatory protein that plays a role in viral transcription and replication. It is a multifunctional protein that is indispensable not only for viral replication but also for evasion of host innate immunity. It establishes a trimeric form when phosphorylated. It is reported to be phosphorylated at serine 162, 210, and 271 by protein kinase C. The trimmer is stabilized by binding to the L protein. It also binds to the soluble protein N, preventing it from encapsidating non-genomic RNA. It is reported to interact with host DYNLL1 and DYNLL2 via amino acids 138-172 and this interaction may play a role in intracellular microtubule-dependent virus transport of incoming virus. It is also reported to inhibits host IFN- and IFN-β signaling by binding and retaining phosphorylated STAT1 in the cytoplasm or by inhibiting the DNA binding of STAT1 in the nucleus. Five isoforms of Rabies virus phosphoprotein have been described that are produced by alternative initiation. Clone M953 binds to the antigenic site consisting of amino acids 37-66 in the native P protein. (Ref.: Okada, K., et al. (2016). J. Virol. 90(18); 8226-8237; Nadin-Davis, SA., et al. (2000). J. Clin. Microbiol. 38(4); 1397-1403).

Spécificité

Clone M953 is a mouse monoclonal antibody that detects phosphoprotein of Rabies virus. It targets an epitope within 30 amino acids from the N-terminal half.

Immunogène

GST-tagged recombinant phosphoprotein from Rabies virus, strain Arctic Fox type I, expressed in E.coli.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Rabies Virus Phosphoprotein.

Western Blotting Analysis: 1:1 million (1.0 ng/mL) dilution of this antibody detected recombinant Rabies Virus Phosphoprotein.

Tested applications

Inhibition Assay: A representative lot of this antibody inhibited binding of Rabies virus phosphoprotein in a competitive binding assay. (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

Immunofluorescence Analysis: A representative lot detected Rabies Virus Phosphoprotein in Immunofluorescence applications (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

ELISA Analysis: A representative lot detected Rabies Virus Phosphoprotein in ELISA applications (Nadin-Davis, S.A., et al. (2000). J Clin Microbiol. 38(4):1397-1403).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rabies Virus Phosphoprotein, clone M953, Cat. No. MABF3151, is a mouse monoclonal antibody that detects Rabies Virus Phosphoprotein and is tested for use in ELISA, Immunofluorescence, Inhibition Assay, and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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