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Merck
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Key Documents

MABF156E

Sigma-Aldrich

Anti-CD4 (Mouse), PE, clone RM4-5 Antibody

clone RM4-5, from rat, PE

Synonyme(s) :

T-cell surface glycoprotein CD4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, CD4, CD4

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702

Source biologique

rat

Niveau de qualité

Conjugué

PE

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

RM4-5, monoclonal

Espèces réactives

mouse

Conditionnement

antibody small pack of 25 μg

Technique(s)

flow cytometry: suitable

Isotype

IgG2aκ

Numéro d'accès UniProt

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Cd4(12504)

Catégories apparentées

Description générale

The RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Anti-CD4 (Mouse), PE, clone RM4-5 Antibody is an antibody against CD4 for use in Flow Cytometry.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Qualité

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected CD4 in one million C57Bl/6 splenocytes.

Forme physique

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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