MABF141B

Anti-MHC class II (I-A/I-E), violetFluor^® 450, clone M5/114 .15.2 Antibody

clone M5/114.15.2, from rat, violetFluor^® 450

• Synonyme(s) : H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain (Ii), MHC class II-associated invariant chain, CD74, MHC class II (I-A/I-E)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Conjugué: violetFluor^® 450
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: M5/114.15.2, monoclonal
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable
• Isotype: IgG2bκ
• Numéro d'accès UniProt: P04441
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Cd74(16149)

Description

Description générale

The M5/114.15.2 antibody reacts with mouse MHC Class II alloantigens I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, as well as being cross-reactive with mouse cells of H-2p and H-2r haplotype. MHC Class II is widely expressed by mouse immune cells bearing these alloantigens, including T and B cells, monocytes, macrophages, and dendritic cells. The antibody does not react with the following alloantigens: I-Af, I-Ak, I-As, or NOD H-2g7The M5/114.15.2 antibody may be used for analysis of mouse cells expressing MHC Class II alloantigens as described. Please note that the M5/114.15.2 clone may also be referred to as M5/114 in the literature.

Immunogène

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rat monoclonal Anti-MHC class II (I-A/I-E) Antibody, violetFluor^® 450, clone M5/114 .15.2, Cat. No. MABF1413 is validated for use in Flow Cytometry, for the detection of MHC class II (I-A/I-E).

Qualité

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 μg from a representative lot detected MHC class II (I-A/I-E) in one million C57Bl/6 splenocytes.

Forme physique

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

violetFluor is a registered trademark of Tonbo Biotechnologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable