MABE979

Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2

clone 2B4.2, from mouse

• Synonyme(s) : Lysine-specific demethylase 5C, Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2B4.2, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001140174
• Numéro d'accès UniProt: P41229
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KDM5C(8242) ; mouse ... Kdm5C(20591)

Description

Description générale

Lysine-specific demethylase 5C (UniProt P41229; also known as Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169) is encoded by the KDM5C (also known as DXS1272E, JARID1C, MRXSCJ, SMCX, XE169) gene (Gene ID 8242) in human. RBP2 (JARID1A), PLU-1 (JARID1B), KDM5C (JARID1C), and SMCY (JARID1D) constitute the Jumonji/ARID (JARID) subfamily of JmjC-containing proteins. KDM5C/JARID1C catalyzes the demethylation of di- and trimethylated lysine 4 of histone 3 in a Fe(II) and α-ketoglutarate-dependent manner. JARID1 catalytic activity is medidated by its JmjC domain (a.a. 468-634), while the JmjN domain (a.a. 14-55) and the C5HC2 zinc finger (a.a. 326-372 & 1187-1248) are both important in assisting JARID1C catalytic activity. JARID1C also contains an AT-rich domain-interacting domain (ARID) that binds DNA and its N-terminal PHD (plant homeodomain) finger (a.a. 326-372) is reported to bind trimethylated lysine 9 on histone 3 (H3K9me3). JARID1C interacts with PCNA through its PCNA-interaction protein (PIP) motif and JARID1C is among 485 chromatin factors enriched at nascent versus mature post-replicative chromatin, indicating a potential transcription-independent role during DNA replication.

Spécificité

Clone 2B4.2 targets an N-terminal region sequence present in all human and murine JARID1C/KDM5C spliced isoforms reported by UniProt (P41229 and P41230).

Immunogène

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding to an N-terminal region sequence of human JARID1C.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

This Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2 is validated for use in Western Blotting for the detection of Histone Demethylase JARID1C.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected histone demethylase JARID1C in 10 µg of HeLa cell lysate.

Description de la cible

~180 kDa observed. 175.7/175.3 kDa (human/mouse isoform 1), 175.4/175.0 kDa (human/mouse isoform 2), 170.6/170.2 kDa (human/mouse isoform 3), 155.1/175.6 kDa (human isoform 4/5) calculated. Uncharacterized band(s) may appear in some lysates.

Forme physique

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1