MABE446

Anti-Histone H1° Antibody, clone 34

clone 34, from mouse

• Synonyme(s) : Histone H1.0, Histone H1(0), Histone H1°
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 34, monoclonal
• Espèces réactives: mouse, bovine, Xenopus, human, rat
• Réactivité de l'espèce (prédite par homologie): ox (immunogen homology)
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001069955
• Numéro d'accès UniProt: Q0IIJ2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... H1F0(3005)

Description

Description générale

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Spécificité

This antibody recognizes Histone H1°.

Immunogène

Recombinant protein corresponding to Ox liver Histone H1°

Application

Anti-Histone H1° Antibody, clone 34 is a highly specific mouse monoclonal antibody, that targets Histone H1 & has been tested in western blotting, ICC, IHC & Flow Cytometry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blotting Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissue lysate (Seigneurin, D., et al. (1995). Int J Dev Biol. 39(4):597-603.; Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.).

Immunocytochemistry Analyis: A representative lot from an independent laboratory detected Histone H1° in Xenopus unfertilized eggs and early embryos (Fu, G., et al. (2003). Biol Reprod. 68(5):1569-1576.; Adenot, P. G., et al. (2000). J Cell Sci. 113(Pt 16):2897-2907.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H1° in Xenopus embryo tissues (Grunwald, D., et al. (1995). Exp Cell Res. 218(2):586-595.).

Flow Cytometry Analyisis: A representative lot from an independent laboratory detected Histone H1° in FC (Grunwald, D., et al. (1999). Methods Mol Biol. 119:443-454.).

Qualité

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H1° in 10 µg of Jurkat cell lysate.

Description de la cible

~30 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Jurkat cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable