MABE348

Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

clone 1G7, from rat

• Synonyme(s) : DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1G7, monoclonal
• Espèces réactives: mouse, yeast, human
• Technique(s): ChIP: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_000928
• Numéro d'accès UniProt: P24928
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pThr4)
• Informations sur le gène: human ... POLR2A(5430)

Description

Description générale

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Spécificité

This antibody recognizes RNAPII phosphorylated at Thr4.

Immunogène

Epitope: Phosphorylated Thr4

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Thr4.

Application

Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cell extracts (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cells (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Thr4) in stably transfected Raji cell lysates (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

Evaluated by Western Blot in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-RNAPII (Thr4) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)

Description de la cible

~220 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Untreated and lambda phosphatase treated HeLa cell lysates.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable