MABE2044

Anti-SPIB Antibody, clone 235D

• Synonyme(s) : Transcription factor Spi-B
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 235D, monoclonal
• Poids mol.: calculated mol wt 28.82 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG1
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_001230927
• Numéro d'accès UniProt: Q01892
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SPIB(6689)

Description

Description générale

Transcription factor Spi-B (UniProt: Q01892; also known as SPIB) is encoded by the SPIB gene (Gene ID: 6689) in human. SPIB is a member of the ETS family of transcription factors that is expressed in mature B cells, T-cell progenitors, and in plasmacytoid dendritic cells. It is reported to be a key regulator of human plasmacytoid dendritic cell development. It may also be required for B-cell receptor (BCR) signaling that is essential for normal B-cell development and antigenic stimulation. It serves as a sequence specific transcriptional activator that binds to the PU-box, a purine-rich DNA sequence (5′-GAGGAA-3′) that can act as a lymphoid-specific enhancer. SPIB contains a weakly acidic N-terminal transactivation domain (TAD; aa 1-31) that is followed by a second TAD (aa 41-61) that is rich in proline, serine, and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes. Overexpression of SPIB is reported in all cases of blastic plasmacytoid dendritic cell (BPDC) neoplasms, but not in its cutaneous mimics. It can be used as a novel immunohistochemical marker for human BPDC neoplasm. (Ref.: Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-647).

Spécificité

Clone 235D is a mouse monoclonal antibody that detects Transcription factor Spi-B (SPIB).

Immunogène

His-tagged recombinant human transcription factor Spi-B (SPIB).

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected SPIB in human tonsil tissue sections.

Tested Applications

Western Blotting Analysis: A representative lot detected SPIB in Western Blotting application (Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-7).

Immunohistochemistry (Paraffin) Analysis (IHC(P)): A representative lot detected SPIB in Immunohistochemistry application (Montes-Moreno, S., et al. (2013). Blood. 121(4); 643-7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SPIB, clone 235D, Cat. No. MABE2044, is a mouse monoclonal antibody that detects transcription factor Spi-B (SPIB) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable