MAB5232
Anti-Presenilin-1 Antibody, loop, a.a. 263-378, CT, clone PS1-loop
ascites fluid, clone PS1-loop, Chemicon®
Synonyme(s) :
Anti-ACNINV3, Anti-AD3, Anti-FAD, Anti-PS1, Anti-S182
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
PS1-loop, monoclonal
Espèces réactives
rat, primate, mouse, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PSEN1(5663)
Description générale
Presenilin is a probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilin requires the other members of the gamma-secretase complex to have a protease activity. It may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. The gamma-secretase complex stimulates cell-cell adhesion through its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, it cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. Futher researcher may implicate the complex in hematopoiesis. Defects in PSEN1 have been implicated in early-onset Alzheimer disease type 3 (AD3) and late-onset Alzheimer disease (LOAD).
Spécificité
Presenilin-1, loop
Immunogène
Epitope: C-terminus
Presenilin-1, loop fusion protein (amino acids 263-378), human
Application
Anti-Presenilin-1 Antibody, loop, a.a. 263-378, C-terminus, clone PS1-loop detects level of Presenilin-1 & has been published & validated for use in IC, IH(P), IP & WB.
Immunocytochemistry:
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).
Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).
Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).
Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Neurodegenerative Diseases
Qualité
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.
Description de la cible
18-20 kDa
Forme physique
Mouse monoclonal IgG1 ascites with no preservatives.
Unpurified
Stockage et stabilité
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
Brain tissue
Brain tissue
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Structure of the catalytic pore of gamma-secretase probed by the accessibility of substituted cysteines.
Sato, C; Morohashi, Y; Tomita, T; Iwatsubo, T
The Journal of Neuroscience null
Mammalian APH-1 interacts with presenilin and nicastrin and is required for intramembrane proteolysis of amyloid-beta precursor protein and Notch.
Lee, SF; Shah, S; Li, H; Yu, C; Han, W; Yu, G
The Journal of Biological Chemistry null
Presenilin-1 regulates induction of hypoxia inducible factor-1?: altered activation by a mutation associated with familial Alzheimer's disease.
De Gasperi, R; Sosa, MA; Dracheva, S; Elder, GA
Mol. Neurodegener. null
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Mark R Farmery et al.
The Journal of biological chemistry, 278(27), 24277-24284 (2003-04-17)
One characteristic feature of Alzheimer's disease is the deposition of amyloid beta-peptide (Abeta) as amyloid plaques within specific regions of the human brain. Abeta is derived from the amyloid beta-peptide precursor protein (beta-APP) by the intramembranous cleavage activity of gamma-secretase.
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