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MAB4081

Sigma-Aldrich

Anti-MnSOD Antibody, clone MnS-1

clone MnS-1, Chemicon®, from mouse

Synonyme(s) :

MnSOD

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

MnS-1, monoclonal

Espèces réactives

rat, rabbit, human

Ne doit pas réagir avec

mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SOD2(6648)

Spécificité

Antibody reacts with MnSOD

Application

Immunohistochemistry on acetone fixed cryostat sections: 0.5-10 μg/mL using an APAAP or PAP procedure. Antibody also reacts with 4% PFA fixed frozen tissue. Typical block contains 1% non-fat dry milk or 3% NGS and 0.2-1.5% triton X-100 {higher triton is only used on thick sections; blocked for 1-3 hours at room temperature. Primary antibody is diluted only in PBS or TBS without excess blocking materials (no triton or milk); typical dilution is 1:200-1:1000 with incubations up to 48 hours at 4C. Washed 3x10′ in PBS and detect via DAB. Immunoreactive neurons are characterized by the dark brown, fine granular deposition in soma and proximal dendrites.

Immunocytochemistry on cell smears: 0.5-10 μg/mL.

Western blot: MnS1 antibody will recognize at 25kDa band under reducing conditions; this is the monomer of the MnSOD protein.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
This Anti-MnSOD Antibody, clone MnS-1 is validated for use in WB, IC, IH for the detection of MnSOD.

Forme physique

Format: Purified
Purified immunoglobulin. Liquid in PBS.

Stockage et stabilité

Prior to opening maintain at 2-8°C for up to six month after date of receipt. After opening maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Aleksandra Jankovic et al.
Free radical research, 50(sup1), S51-S63 (2016-09-07)
Setting the correct ratio of superoxide anion (O2•-) and nitric oxide (•NO) radicals seems to be crucial in restoring disrupted redox signaling in diabetic skin and improvement of •NO physiological action for prevention and treatment of skin injuries in diabetes.
Proteomic analysis of radiation-induced changes in rat lung: Modulation by the superoxide dismutase mimetic MnTE-2-PyP(5+).
Yakovlev, VA; Rabender, CS; Sankala, H; Gauter-Fleckenstein, B; Fleckenstein et al.
International Journal of Radiation Oncology, Biology, Physics null
Wafa Kammouni et al.
Journal of virology, 86(15), 8139-8146 (2012-05-25)
Recent studies in an experimental model of rabies showed major structural changes in the brain involving neuronal processes that are associated with severe clinical disease. Cultured adult rat dorsal root ganglion (DRG) neurons infected with the challenge virus standard-11 strain
Emily E Brown et al.
Redox biology, 24, 101201-101201 (2019-05-01)
Age-related macular degeneration (AMD) is the leading cause of vision loss in the western world. Recent evidence suggests that RPE and photoreceptors have an interconnected metabolism and that mitochondrial damage in RPE is a trigger for degeneration in both RPE
Phatthamon Laphanuwat et al.
Journal of cell science, 131(12) (2018-06-09)
Expression of cyclin D1 (CCND1) is required for cancer cell survival and proliferation. This is presumably due to the role of cyclin D1 in inactivation of the RB tumor suppressor. Here, we investigated the pro-survival function of cyclin D1 in

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