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MAB3136

Sigma-Aldrich

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5

clone 4F5, Chemicon®, from mouse

Synonyme(s) :

NHE3

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

4F5, monoclonal

Espèces réactives

rat, rabbit

Fabricant/nom de marque

Chemicon®

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_001082697.1

Numéro d'accès UniProt

P26432

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

rabbit ... Slc9A3(100009430)
rat ... Slc9A3(24784)

Spécificité

Na+/H+ exchanger, isoform NHE3.

Immunogène

Epitope: isoform NHE3
Maltose Binding Protein fusion protein containing C-terminal 131 amino acids of rabbit NHE3.

Application

Anti-Na+/H+ Exchanger-3 Antibody, isoform NHE3, clone 4F5 detects level of Na+/H+ Exchanger-3 & has been published & validated for use in IC, IH(P), IP & WB.
Immunoblotting (rabbit 80 kDa and rat ~83 kDa)

Immunohistochemistry (rat) on PLP or paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections.

Immunocytochemistry

Immunoprecipitation (use non-ionic detergent)

Optimal working dilutions must be determined by the user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Liaison

Replaces: MAB3134

Forme physique

Format: Purified
Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Distribution of pH regulators in the rat laryngeal nerve: the spatial relationship between Na(+)/HCO(3)(-) cotransporters and Na(+)/H(+) exchanger type 3.
Yoshio Yamamoto, Kazuyuki Taniguchi
Neuroscience Letters null
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney.
Biemesderfer, D, et al.
The American Journal of Physiology, 273, F289-F299 (1997)
Yanling Yan et al.
Molecular and cellular biochemistry, 367(1-2), 175-183 (2012-05-24)
We have demonstrated that ouabain regulates protein trafficking of the Na/K-ATPase α1 subunit and NHE3 (Na/H exchanger, isoform 3) via ouabain-activated Na/K-ATPase signaling in porcine LLC-PK1 cells. To investigate whether this mechanism is species-specific, ouabain-induced regulation of the α1 subunit
Lactobacillus acidophilus upregulates intestinal NHE3 expression and function.
Singh, V; Raheja, G; Borthakur, A; Kumar, A; Gill, RK; Alakkam, A; Malakooti, J; Dudeja, PK
American Journal of Physiology: Gastrointestinal and Liver Physiology null
Expression and localization of the Na+/H+ exchanger isoform NHE3 in the rat efferent ducts.
Kari M Kaunisto, Hannu J Rajaniemi
Journal of Andrology null
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