MAB1692
Anti-Dystrophin Antibody, mid-rod, clone 6D3
culture supernatant, clone 6D3, Chemicon®
Synonyme(s) :
Anti-BMD, Anti-CMD3B, Anti-DXS142, Anti-DXS164, Anti-DXS206, Anti-DXS230, Anti-DXS239, Anti-DXS268, Anti-DXS269, Anti-DXS270, Anti-DXS272, Anti-MRX85
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
6D3, monoclonal
Espèces réactives
mouse, canine, human, rabbit, rat
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2a
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... DMD(1756)
Spécificité
Mid rod domain (between amino acids 1181 and 1388) of human dystrophin. Also reacts with skeletal, cardiac and smooth muscle dystrophin from normal mouse, rat, rabbit and dog. Other animal species have not been tested. Reacts on blots with the brain isoform. No reactivity with mdx mouse tissue or DMD/BMD patients who have a gene deletion which removes the antibody binding site. Does not react with chicken dystrophin.
STAINING PATTERN:Light microscopy: continuous rim of labeling at the periphery of muscle fibers.
E.M. gold: close to the cytoplasmic face of the plasma membrane.
Western blotting: strong double bands at approximately 400 kD plus metabolites of lower molecular mass.
STAINING PATTERN:Light microscopy: continuous rim of labeling at the periphery of muscle fibers.
E.M. gold: close to the cytoplasmic face of the plasma membrane.
Western blotting: strong double bands at approximately 400 kD plus metabolites of lower molecular mass.
Immunogène
Bacterial fusion protein (Cell (1987) 51:919-928).
Epitope: mid-rod
Application
Immunohistochemistry: (fresh frozen, unfixed tissue only): use
undiluted - 1:20. Not recommended for use on paraffin embedded tissue.
EM Gold (Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. 2.3M sucrose used as cryoprotectant.): use undiluted. 90 minute incubation at 25°C.
Western blotting: use 1:100-1:250.
Optimal working dilutions must be determined by the end user.
Protocol for Immunohistochemical use of MAB1692
1) Freeze muscle blocks in isopentane chilled in liquid nitrogen.
2) Cut 4 μm to 10 μm sections and air dry on slides coated with 0.5% gelatin containing 0.05% chrome alum.
3) Slides may be stored at -70 °C wrapped in cling film until required. If stored sections are used, allow sections to equilibrate to room temperature before unwrapping and proceeding.
4) Apply a 50 μL aliquot of primary antibody to sections (unfixed). Incubate for 1 hour at room temperature or 37°C.
5) Wash sections 3 x 10 minutes in phosphate buffered saline.
6) Apply a 50 μL aliquot of labeled second antibody. Incubate for 60 minutes at 25°C.
7) Wash sections 3 x 10 minutes in phosphate buffered saline.
8) Mount fluorescent sections in aqueous mounting media or visualize peroxidase label (DAB). Dehydrate, clean and mount peroxidase labeled sections for permanent preparations.
undiluted - 1:20. Not recommended for use on paraffin embedded tissue.
EM Gold (Light fixation with 2% formaldehyde + 0.001% glutaraldehyde for 1 hour. 2.3M sucrose used as cryoprotectant.): use undiluted. 90 minute incubation at 25°C.
Western blotting: use 1:100-1:250.
Optimal working dilutions must be determined by the end user.
Protocol for Immunohistochemical use of MAB1692
1) Freeze muscle blocks in isopentane chilled in liquid nitrogen.
2) Cut 4 μm to 10 μm sections and air dry on slides coated with 0.5% gelatin containing 0.05% chrome alum.
3) Slides may be stored at -70 °C wrapped in cling film until required. If stored sections are used, allow sections to equilibrate to room temperature before unwrapping and proceeding.
4) Apply a 50 μL aliquot of primary antibody to sections (unfixed). Incubate for 1 hour at room temperature or 37°C.
5) Wash sections 3 x 10 minutes in phosphate buffered saline.
6) Apply a 50 μL aliquot of labeled second antibody. Incubate for 60 minutes at 25°C.
7) Wash sections 3 x 10 minutes in phosphate buffered saline.
8) Mount fluorescent sections in aqueous mounting media or visualize peroxidase label (DAB). Dehydrate, clean and mount peroxidase labeled sections for permanent preparations.
Research Category
Metabolism
Metabolism
Research Sub Category
Muscle Physiology
Muscle Physiology
This Anti-Dystrophin Antibody, mid-rod, clone 6D3 is validated for use in WB, IH for the detection of Dystrophin.
Forme physique
Culture supernatant, liquid in PBS with 1% BSA, containing 15 mM sodium azide.
Stockage et stabilité
Maintain at -20°C for up to one year in convenient undiluted aliquots. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
POSITIVE CONTROL: Snap frozen normal human or rat striated muscle.
POSITIVE CONTROL: Snap frozen normal human or rat striated muscle.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Heterogeneity of dystrophin expression in patients with Duchenne and Becker muscular dystrophy.
Nicholson, L V, et al.
Acta neuropathologica, 80, 239-250 (1990)
A deficit of brain dystrophin impairs specific amygdala GABAergic transmission and enhances defensive behaviour in mice.
Sekiguchi, M; Zushida, K; Yoshida, M; Maekawa, M; Kamichi, S; Yoshida, M; Sahara et al.
Brain null
A B-Myb complex containing clathrin and filamin is required for mitotic spindle function.
Tomohiro Yamauchi,Takefumi Ishidao,Teruaki Nomura,Toshie Shinagawa,Yasunori Tanaka et al.
The Embo Journal null
S Masuda et al.
Acta physiologica (Oxford, England), 195(4), 483-494 (2008-12-02)
The dystrophin-glycoprotein complex (DGC) and focal adhesion complex (FAC) are transmembrane structures in muscle fibres that link the intracellular cytoskeleton to the extracellular matrix. DGC and FAC proteins are abundant in slow-type muscles, indicating the structural reinforcement which play a
Is dystrophin labelling always discontinuous in Becker muscular dystrophy?
Slater, C R and Nicholson, L V
Journal of the Neurological Sciences, 101, 187-192 (1991)
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