MAB1620
Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4
clone D5/16B4, Chemicon®, from mouse
Synonyme(s) :
58 kDa cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II keratin Kb5
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Clone
D5/16B4, monoclonal
Espèces réactives
rat, mouse, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... KRT5(3852)
Description générale
The cytokeratins are a family of intermediate filaments found in almost all epithelial cells. They are divided into four subclasses depending on their patterns of reactivity and electrophoretic characteristics and are also arranged I a numbered series of 20 or more acoring to molecular weight and isoelectric point (Quinlan et al. 1985). Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen′s disease. It does not react with pulmonary adenocarcinoma.
Spécificité
Expected to cross-react with mouse and rat.
Reacts with human cytokeratin 5 on immunoblots of cytoskeletal preparations of epidermis and non-cytokeratinizing epithelium (Abstract: European Symposium of the Biology of the Cytoskeleton, Helsinki, 18-21, 6, 89, 1989) and on tissue sections. Shows reactivity with cytokeratin 6 and weak reactivity with cytokeratin 4 on immunoblots. No cross-reactivity with cytokeratins 1, 7, 8, 10, 13, 14, 18 or 19. MAB1620 recognizes basal cells and a part of the stratum spinosum in the normal pavement epithelium. The antibody may be useful for distinction of low differentiated pavement epithelium carcinoma and adenocarcinoma. The antibody works on formalin fixed tissues.
Immunogène
Purified cytokeratin 5 (Exp. Cell Res. (1986) 162:114; J. Invest. Dermatology (1987) 88:191).
Application
Anti-Cytokeratin 5 Antibody, 6, clone D5/16B4 is an antibody against Cytokeratin 5 for use in IF, IH(P) & WB.
Immunohistochemistry (frozen or paraffin sections):
A 1:50-1:100 dilution from a previous lot was used for paraffin, high-temperature antigen retrieval required (Pressure cooker, Citrate/EDTA buffer pH 6.0, 4 minutes at full pressure).
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Western blot:
Recognizes a 58kDa and 56kDa band corresponding to cytokeratin 5 and 6.
Optimal working dilutions must be determined by end user.
A 1:50-1:100 dilution from a previous lot was used for paraffin, high-temperature antigen retrieval required (Pressure cooker, Citrate/EDTA buffer pH 6.0, 4 minutes at full pressure).
Immunofluorescence:
A previous lot of this antibody was used in Immunofluorescence.
Western blot:
Recognizes a 58kDa and 56kDa band corresponding to cytokeratin 5 and 6.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Qualité
Routinely evaluated by Western Blot on A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected cytokeratin 5/6 on 10 ug of A431 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected cytokeratin 5/6 on 10 ug of A431 lysates.
Description de la cible
Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).
Forme physique
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.
Stockage et stabilité
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Remarque sur l'analyse
Control
A431 cell lysate, human bladder tumor tissue.
A431 cell lysate, human bladder tumor tissue.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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