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HMYOMAG-56K

Millipore

MILLIPLEX® Human Myokine Magnetic Bead Panel

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84

Niveau de qualité

Espèces réactives

human

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 100%
(Apelin)

accuracy: 92%
(IL-15)

accuracy: 96%
(FSTL-1)

accuracy: 97%
(LIF)

accuracy: 98%
(Oncostatin M (OSM))

sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)

sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)

sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)

sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)

sensitivity: 4 pg/mL
(MinDC+2SD; LIF)

sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)

sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)

sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)

sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))

sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)

sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))

sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))

sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))

sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)

sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)

standard curve range: 0.6-2,400 pg/mL
(IL-6)

standard curve range: 10-40,000 pg/mL
(LIF)

standard curve range: 122-500,000 pg/mL
(Apelin)

standard curve range: 2-10,000 pg/mL
(BDNF)

standard curve range: 2-10,000 pg/mL
(IL-15)

standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))

standard curve range: 24-100,000 pg/mL
(FABP3)

standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))

standard curve range: 244-1,000,000 pg/mL
(Irisin)

standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))

standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)

standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)

standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)

standard curve range: 5-20,000 pg/mL
(FGF-21)

standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))

inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)

inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)

inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))

inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)

inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)

inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))

inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)

inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)

inter-assay cv: <15%
intra-assay cv: <10%
(LIF)

inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)

inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))

inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)

inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Description générale

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems.

MILLIPLEX® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

  • Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
  • Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
  • NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
  • Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
  • Research Category: Bone
  • Research Subcategory: Metabolism

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Autres remarques

Please contact Technical Service for linearity of dilution.

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2

Organes cibles

Respiratory Tract

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Kelly Ann Hutchinson et al.
Frontiers in physiology, 10, 1188-1188 (2019-10-28)
It is recommended that women accumulate 150-min of weekly moderate-intensity physical activity (MPA) when pregnant. Engaging in regular physical activity (PA) confers many health benefits to both the mother and the fetus. However, the molecular mechanisms by which these health
Javier Diaz-Castro et al.
Frontiers in nutrition, 8, 761213-761213 (2021-11-09)
Practicing exercise is one of the best strategies to promote well-being and quality of life, however physical activity in schoolchildren and adolescents is developed in an unpredictable, intermittent way and in short periods. There are relatively few intervention studies investigating

Contenu apparenté

Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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