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EZIL6

Sigma-Aldrich

Human IL-6 ELISA Kit

detect IL-6 in 50 μL serum, plasma or cell culture supernatent

Synonyme(s) :

B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IL6, Interferon beta-2, Interleukin-6

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.77

product name

Human IL-6 Conferma® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples

Niveau de qualité

Espèces réactives

human

Paramètres

50 μL sample volume (4hr assay)

assay range

linearity: 105-116%
(serum)

linearity: 88-105%
(plasma)

inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%

Technique(s)

ELISA: suitable

Entrée

sample type serum
sample type plasma (K2 EDTA)
sample type cell culture supernatant

Numéro d'accès UniProt

Application(s)

research use

Méthode de détection

colorimetric (450nm/590nm)

Informations sur le gène

human ... IL6(3569)

Description générale

This Human Interleukin-6 (IL-6) Conferma® ELISA kit is used for the quantification of Human IL-6 in serum, plasma, or culture media samples. Human IL-6 is a soluble protein cytokine most commonly associated with the acute inflammatory response to infection or tissue damage.

This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs

Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.

UniProt Summary:
  • Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
  • Size: 212 amino acids; 23,718 Da
  • Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
  • Subcellular location: Extracellular region or secreted
  • Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Similarity: Belongs to the IL-6 superfamily.

Application

One kit is sufficient to measure 37 samples in duplicate

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Remarque sur l'analyse

3 hour 45 minute assay, room temperature, 50 μL sample size (serum, plasma, or tissue culture medium)

Informations légales

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

For research use only. Not for use in diagnostic procedures.

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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