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AC111B

Sigma-Aldrich

Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate

from sheep, biotin conjugate

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.46

Source biologique

sheep

Niveau de qualité

Conjugué

biotin conjugate

Forme d'anticorps

purified antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Espèces réactives

mouse

Technique(s)

immunohistochemistry: suitable

Isotype

IgG1

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Description générale

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Spécificité

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

Immunogène

Purified mouse IgG.

Application

Immunohistochemistry Analysis: A representative lot was validated by immunohistochemistry staining (1:500 dilution) of human liver tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate is an antibody against Sheep Mouse IgG for use in Immunohistochemistry.

Qualité

Evaluated by Immunohistochemistry in human prostate tissue.

Immunohistochemistry Analysis: Performance of this biotin-conjugated secondary antibody has been validated by immunohistochemistry staining (1:500 dilution) of human prostate tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).

Description de la cible

~150 kDa observed.

Forme physique

Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to biotin conjugation.
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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