ABS2131M

Anti-phospho Rab1 (Thr75)

from rabbit

• Synonyme(s) : YPT-1 related protein, Ras-related protein Rab-1A
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), sheep (based on 100% sequence homology), rat (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μL
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_004152
• Numéro d'accès UniProt: P62820
• Modification post-traductionnelle de la cible: phosphorylation (pThr75)
• Informations sur le gène: human ... RAB1A(5861)

Description

Description générale

Ras-related protein Rab-1A (UniProt: P62820; also known as YPT1-related protein) is encoded by the RAB1A (also known as RAB1) gene (Gene ID: 5861) in human. Rab1 is a member of the small GTPase superfamily. Rab proteins are the largest family of small Ras-like GTPases. Three isoforms of Rab1 have been reported that are produced by alternative splicing. Rab GTPases are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. They cycle between an inactive GDP-bound form and an active GTP-bound form, which is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering, and fusion. Rab1 is reported to regulate vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface. Rab1 is also shown to be involved in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Rab1 is shown to be phosphorylated at Thr75 by TGF-beta activated kinase 1 (TAK1), which preferentially phosphorylates the inactive (GDP-bound) state of Rab1. Phosphorylation disrupts interaction with GDP dissociation inhibitor 1 (GDI1), but not GEF or GAP. Phosphorylation at Thr75 is shown to be essential for Rab1 function.

Spécificité

This rabbit polyclonal antibody detects Ras-related protein Rab-1A (Rab1) in human cells. It targets an epitope within 14 amino acids surrounding phosphorylated Thr75.

Immunogène

A linear peptide corresponding to 14 amino acids surrounding phosphorylated Thr75 from the N-terminal half of human Ras-related protein Rab-1A.

Epitope: unknown

Application

Anti-phospho Rab1 (Thr75), Cat. No. ABS2131, is a rabbit polyclonal antibody that detects Ras-related protein Rab-1A and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected phospho Rab1 (Thr75) in Western Blotting applications (Levin, R.S., et. al. (2016). Proc Natl Acad Sci USA. 113(33):E4776-83).

Qualité

Evaluated by Western Blotting in lysate from HEK293T stably expressing GST-Rab1a-Tak1.


Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho Rab1 (Thr75) in lysates from HEK-293T stably expressing GST-Rab1a-Tak1.

Description de la cible

~48 kDa observed; 22.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2