ABN1720
Anti-Ninein
serum, from rabbit
Synonyme(s) :
Ninein, Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein
About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
rat, human, mouse
Technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Nin(18080)
Description générale
Spécificité
Immunogène
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cytoplasmic and centrosome ninein immunoreactivity in 0.1 Triton X-100 extracted and 4% paraformaldehyde-fixed HEK293T cells (Courtesy of Professor Kensuke Hayashi, Sophia Univesity, Japan).
Immunofluorescence Analysis: A representative lot detected ninein immunoreactivity in the centrosome of proliferative apical progenitor cells (APs) by fluorescent immunohistochemisty staining of 4% paraformaldehyde-fixed cerebral cortex cryosections from E17.5 rat embryos. Ninein immunoreactivity was found downregulated in embryo sections from small eye rats with homozygous rSey2/rSey2 Pax6 mutation (Shinohara, H., et al. (2013). Biol Open. 2(7):739-749).
Immunofluorescence Analysis: A representative lot detected intense ninein immunoreactivity at the ventricular surface by fluorescent immunohistochemisty staining of 4% paraformaldehyde-fixed cerebral cortex cryosections from E13 and E18 mouse embryos. Lower immunoreactivity was also observed in the E18 cortical plate (CP), but was of much weaker intensity in the E13 cortex (Ohama, Y., and Hayashi, K. (2009). Histochem. Cell Biol. 132(5):515-524).
Immunocytochemistry Analysis: A representative lot detected granular ninein immunoreactivity in somatodendritic compartment, but not in the axon by fluorescent immunocytochemistry staining of 4% paraformaldehyde-fixed cultured cortical neurons from E18 mouse embryos. About 60% of ninein granules are associated with microtubules and showed resistance to 0.5% Triton X-100 (Ohama, Y., and Hayashi, K. (2009). Histochem. Cell Biol. 132(5):515-524).
Immunohistochemistry Analysis: A representative lot detected ninein immunoreactivity in the cortical plate (CP), white matter (WM), and the ventricular zone (VZ) of paraffin-embedded embryonic E16.5 mouse brain sections. Ninein immunoreactivity was found downregulated in the CP and WM, but not VZ, of Sip1fl/flNexCre embryos with hippocampal granule cell-targeted Sip1 knockout (Srivatsa, S., et al. (2015). Neuron 85(5):998-1012).
Western Blotting Analysis: A representative lot detected ninein in cortical lysate from postnatal P0, P3, P4 and P7 mouse brain tissue homogenates (Srivatsa, S., et al. (2015). Neuron 85(5):998-1012).
Note: Extracting soluble ninein under microtubule-stabilizing conditions with 0.5% Triton X-100 in the presence of 10 M paclitaxel (Cat. Nos. 580555 & 580556) prior to fixation can be employed to better visualize microtubule- and centrosome-associated ninein immunoreactivity (Ohama, Y., and Hayashi, K. (2009). Histochem. Cell Biol. 132(5):515-524).
Qualité
Immunocytochemistry Analysis (ICC): A 1:500 dilution of this antiserum detected ninein in NIH/3T3 cells..
Description de la cible
Autres remarques
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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