ABF185

Anti-Properdin Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Complement factor P, Properdin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse, rat
• Réactivité de l'espèce (prédite par homologie): nonhuman primates (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_002612
• Numéro d'accès UniProt: P27918
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CFP(5199)

Description

Description générale

Properdin (UniProt P27918; also known as Complement factor P) is encoded by the CFP (also known as PFC, PFD) gene (Gene ID 5199) in human. Properdin is a plasma glycoprotein and the only known positive regulator of the complement cascade. It binds and stabilizes the alternative pathway (AP) C3 convertase C3bBb and may also serve as a platform to form new C3bBb convertases on cell surface. Properdin-mediated AP activation plays an important role in host defense, and properdin deficiency (PFD) increases the risk for Neisseria meningitidis infection in human. Properdin-deficiency exacerbated disease condition in a murine model of factor H- (fH-) related C3 glomerulopathy, but prevented the development of allergen-induced airway inflammation. Human properdin is produced with a signal peptide sequence (a.a. 1-27), which is removed posttranslationally to yield the mature protein (a.a. 28-469) composed entirely of seven TSP type-1 domains (domain 0 through 6; a.a. 28-462). TSP type-1 domains 0 and 6 bind to each other and mediate multimerization.

Spécificité

This polyclonal antibody detects properdin by targeting a sequence in the fourth TSP type-1 domain (TSP type-1 domain 3).

Immunogène

Epitope: Within TSP type-1 domain 3.

KLH-conjugated linear peptide corresponding to a TSP type-1 domain 3 sequence of human Properdin.

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

This Anti-Properdin Antibody is validated for use in Western Blotting for the detection of Properdin.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Properdin in 10 µg of mouse serum.

Qualité

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Properdin in 10 µg of rat serum.

Description de la cible

~52 kDa observed. 48.49/47.94/48.18 kDa (human/mouse/rat mature form) and 51.28/50.33/50.57 kDa (human/mouse/rat pro-form) calculated.

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1