ABF1159

Anti-DEFA1

from rabbit, purified by affinity chromatography

• Synonyme(s) : HP 1-56, Neutrophil defensin HP 1-56 chain
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rhesus macaque (based on 100% sequence homology)
• Technique(s): flow cytometry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_004075
• Numéro d'accès UniProt: P59665
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DEFA1(1667)

Description

Description générale

The human DEFA1 gene (also known as DEF1, DEFA2, MRS, DEFA1B; Gene ID 1667) encodes a 94-amino acid precursor protein (UniProt P59665) with an N-terminal signal peptide (a.a. 1-19) and propeptide (a.a. 20-38) sequence. Posttranslational processing by proteolytic cleavages generates three neutrophil granule peptides, HP 1-56 (a.a. 39-94), neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94), and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94). These peptides exhibit several biological activities including the non-oxidative destruction of ingested microorganisms, the inhibition of adrenocorticotropin-stimulated glucocorticoids synthesis, monocyte chemotaxis, the non-cytolytic inhibition of thymidine incorporation in HL-60 promyelocyte-like cells and the stimulation of nifedipine-sensitive calcium channels. The richest source of HP1-56 relative to HP-1 is found in fetal lung tissue. In addition, HP1-56 is found in the HL-60 promyelocytic cell line and in some leukemias, but not in normal mature circulating neutrophils.

Spécificité

This rabbit polyclonal antibody targets a region present in prepro-, pro-, and mature HP 1-56 (a.a. 1-94, 20-94, and 39-94, respectively). Target region is absent from neutrophil defensin 1 (HNP-1, HP-1, HP1; a.a. 65-94) and neutrophil defensin 2 (HNP-2, HP-2, HP2; a.a. 66-94).

Immunogène

KLH-conjuated linear peptide corresponding to an internal sequence of human HP 1-56.

Application

Flow Cytometry Analysis: 0.1 µL from a representative lot detected HP 1-56 immunoreactivity in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 human promyelocytic leukemia cells.

Research Category
Inflammation & Immunology

This rabbit polyclonal Anti-DEFA1 Antibody, Cat. No. ABF1159 is validated for use in Flow Cytometry and Western Blotting for the detection of HP 1-56.

Qualité

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:5000 dilution of this antibody detected HP 1-56 in 10 µg of HL-60 human promyelocytic leukemia cell lysate.

Description de la cible

~10 kDa observed. 10.20/8.238/6.306 kDa (prepro-/pro-/mature HP 1-56) calculted. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable