ABF1070

Anti-DRBP76/ILF3 Antibody

serum, from rabbit

• Synonyme(s) : Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, DRBP76, M-phase phosphoprotein 4, MPP4, Nuclear factor associated with dsRNA, NFAR, Nuclear factor of activated T-cells 90 kDa, NF-AT-90, Translational control protein 80, TCP
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NP_036350
• Numéro d'accès UniProt: Q12906
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ILF3(3609)

Description

Description générale

Interleukin enhancer-binding factor 3 (UniProt Q12906; also known as Double-stranded RNA-binding protein 76, DRBP76, dsRNA binding protein NFAR-2/MPP4, Interleukin enhancer binding factor 3 90kD, M-phase phosphoprotein 4, MPP4, NF-AT-90, NFAR, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, TCP80, Translational control protein 80) is encoded by the ILF3 (also known as DRBF, NF90, MPHOSPH4) gene (Gene ID 3609) in human. DRBP76 is a dsRNA binding protein (DRBP) family member capable of binding both dsRNAs and highly structured single-stranded RNA molecules through its C-terminal region. DRBP76 influences the nucleus-cytoplasm export, stability, and translation of target mRNAs via direct interaction with their 3′ UTRs. DRBP76 is also reported to play both positive and negative regulatory roles in viral replications. DRBP76 is shown to interact with the 3′-terminal stem loop (SL) structure of the single-stranded RNA genome of the dengue virus and DRBP76 knockdown decreases production of functional dengue virus in cultured cells. On the other hand, DRBP76 can interact with full-length Zaire Ebola virus (EBOV) protein VP35 in the absence of exogenous dsRNA. Viral VP35 expression redistributes DRBP76 from the nucleus to the cytoplasm in the host cells and over-expression of DRBP76 impairs the function of the EBOV transcription/replication complex.

Spécificité

Expected to react with all 7 spliced isoforms.

Immunogène

His-tagged recombinant protein corresponding to human DRBP76/ILF3.

Application

This Anti-DRBP76/ILF3 Antibody is validated for use in Western Blotting for the detection of DRBP76/ILF3.

Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from the squamous cell carcinoma of the head and neck (SCCHN) cell lines PCI-15A and PCI-15B (Umemura, N., et al, (2012). Cancer Res. 72(1):45-55).
Western Blotting Analysis: A representative lot detected DRBP76/ILF3 in the nuclear fractions prepared from Wt11 (HEK293 expressing Flag-tagged wild-type TLR3) cells (Zhu, J., et al, (2010). J Immunol. 184(10):5768-5776; Sarkar, S.N., et al, (2004). Nat Struct Mol Biol. 11(11):1060-1067).

Qualité

Evaluated by Western Blotting in MDA-MB-435 cell lysate.

Western Blotting Analysis: A 1:500 of this antibody detected DRBP76/ILF3 in 10 µg of MDA-MB-435 cell lysate.

Description de la cible

~100 kDa A lower size band ~75 kDa may be seen in some lysates, most likely representing a smaller size variant or a non-specific band. Uncharacterized band(s) may appear in some lysates.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable