Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

ABE569

Sigma-Aldrich

Anti-monoubiquitin H2A Antibody (Lys119)

from rabbit, purified by affinity chromatography

Synonyme(s) :

H2AK119ub1, Histone H2A.1, Histone H2A.2, H2A2, h2aUb

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human, mouse

Technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Numéro d'accès NCBI

XP_001021342

Numéro d'accès UniProt

P0C0S8

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

rat ... Hist2H2Aa2(690131)

Description générale

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The ubiquitination of Histones plays essential roles in the regulation of DNA related processes.

Immunogène

Branch peptide corresponding to human monoubiquitin H2A (Lys119).

Application

Detect Histone H2A using this rabbit polyclonal antibody, Anti-monoubiquitin H2A Antibody (Lys119) validated for use in western blotting, ChIP, IP and ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot from an independent laboratory detected monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated monoubiquitin H2A in mouse Embryonic Stem cell lysate (Farcas, A. M. et al. (2012). Elife [Epub ahead of print].).

Qualité

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected monoubiquitin H2A (Lys119) in 10 µg of HeLa acid extract.

Description de la cible

~23 kDa observed. Uniprot describes one isoform at 14 kDa; however, this protein may be observed at ~23 kDa due to monoubiquitination.

Forme physique

Affinity purified
Purified rabbit polyclonal in buffer containing 90 mM Tris-glycine pH8, 50 mM Nacl, and 10% glycerol with 0.05% sodium azide

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Remarque sur l'analyse

Control
HeLa acid extract

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Mei-Jun Zhao et al.
Oncotarget, 7(8), 8956-8967 (2016-01-21)
The ubiquitin ligase RNF8 promotes the DNA damage response (DDR). We observed that the expression of RNF8 was increased in bladder cancer cells and that this change in RNF8 expression could be reversed by adenovirus-mediated shRNA treatment. Moreover, we found
Barbara Dupret et al.
PloS one, 11(7), e0158700-e0158700 (2016-07-22)
Polycomb Repressive Complex (PRC) 1 regulates the control of gene expression programs via chromatin structure reorganization. Through mutual exclusion, different PCGF members generate a variety of PRC1 complexes with potentially distinct cellular functions. In this context, the molecular function of
Fei-Fei Hu et al.
Molecular therapy. Nucleic acids, 27, 670-684 (2022-01-25)
The disruption of epigenetic regulation is common in tumors; the abnormal expression of epigenetic factors leads to cancer occurrence and development. In this study, to investigate the potential function of histone methylation regulators in lung adenocarcinoma (LUAD), we performed differential
KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands.
Farcas, AM; Blackledge, NP; Sudbery, I; Long, HK; McGouran, JF; Rose, NR; Lee, S; Sims et al.
eLife null
Lan-Tao Gou et al.
Cell, 169(6), 1090-1104 (2017-05-30)
Genetic studies have elucidated critical roles of Piwi proteins in germline development in animals, but whether Piwi is an actual disease gene in human infertility remains unknown. We report germline mutations in human Piwi (Hiwi) in patients with azoospermia that prevent its ubiquitination
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique