ABE44
Anti-trimethyl Histone H3 (Lys27) Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
H3K27me3, Histone H3 (tri methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human
Technique(s)
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
trimethylation (Lys27)
Informations sur le gène
human ... HIST1H3F(8968)
Description générale
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 lysine 27 methylation (H3K27) is associated with heterochromatin formation and transcriptional repression.
Spécificité
Broad species cross-reactivity expected.
This antibody recognizes Histone H3 methylated at Lys27.
Immunogène
Branch peptide corresponding to human Histone H3 methylated at Lys27.
Epitope: Lys27
Application
Anti-trimethyl Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody for detection of Histone H3 trimethylated on lysine 27( H3K27me3). This purified polyclonal is specificity verified by dot blot (DB), published in peer reviewed journals and validated in WB, ICC, PIA.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in NIH/3T3 cells.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.
Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Qualité
Evaluated by Western Blot in HeLa acid extract and recombinant Histone H3.
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Western Blot Analysis: 0.1 µg/ml of this antibody detected histone H3 in 10 µg of HeLa acid extract .
Description de la cible
~ 17 kDa
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
HeLa acid extract and recombinant Histone H3
HeLa acid extract and recombinant Histone H3
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Chromatin Signature Identifies Monoallelic Gene Expression Across Mammalian Cell Types.
Nag, A; Vigneau, S; Savova, V; Zwemer, LM; Gimelbrant, AA
G3 (Bethesda, Md.) null
Development of multiple cell-based assays for the detection of histone H3 Lys27 trimethylation (H3K27me3).
Qian, J; Lu, L; Wu, J; Ma, H
Assay and Drug Development Technologies null
Chromatin signature of widespread monoallelic expression.
Nag, A; Savova, V; Fung, HL; Miron, A; Yuan, GC; Zhang, K; Gimelbrant, AA
eLife null
James L Thorne et al.
Nucleic acids research, 39(6), 2045-2056 (2010-11-23)
In non-malignant RWPE-1 prostate epithelial cells signaling by the nuclear receptor Vitamin D Receptor (VDR, NR1I1) induces cell cycle arrest through targets including CDKN1A (encodes p21((waf1/cip1))). VDR dynamically induced individual histone modification patterns at three VDR binding sites (R1, 2
Lei Yang et al.
EMBO reports, 19(12) (2018-11-06)
Despite the success of animal cloning by somatic cell nuclear transfer (SCNT) in many species, the method is limited by its low efficiency. After zygotic genome activation (ZGA) during mouse development, a large number of endogenous retroviruses (ERVs) are expressed
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