ABE1844
Anti-NRF-1 Antibody
from rabbit
Synonyme(s) :
Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
mouse, human, rat
Réactivité de l'espèce (prédite par homologie)
porcine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology), horse (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
Technique(s)
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Nrf1(18181)
Description générale
Nuclear respiratory factor (UniProt Q9WU00; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the Nrf1 gene (also known as C87038, D6Ertd415e) in murine species (Gene ID 18181). NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS). This results in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
Immunogène
KLH-conjugated linear peptide corresponding to mouse NRF-1.
Application
Immunocytochemistry Analysis: A representative lot detected NRF-1 in neonatal rat cardiomyoblasts (H9c2) (Data courtesy of Hagir B. Suliman and Claude A. Piantadosi , Duke University Medical Center).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatoma cell line H4IIE treated with t-BH (Suliman, H.B., et al. (2003). J. Biol. Chem. 278(42):41510-41518).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse hepatic nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse liver after S. aureus inoculation (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatocytes and rat hepatoma cell line H4IIE cells (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).
Chromatin Immunoprecipitation Analysis: A representative lot detected enhanced NRF-1 occupancy at the Sod2 promoter region in liver nuclear extracts from mice during S. aureus sepsis (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in Staphylococcus aureus peptidoglycan
(PGN) or dichloromethane (DCM)/carbon monoxide (CO) treated HepG2 cells (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in H9C2 rat cardiomyocytes (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatoma cell line H4IIE treated with t-BH (Suliman, H.B., et al. (2003). J. Biol. Chem. 278(42):41510-41518).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse hepatic nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Western Blotting Analysis: A representative lot detected NRF-1 in mouse liver after S. aureus inoculation (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Western Blotting Analysis: A representative lot detected NRF-1 in rat hepatocytes and rat hepatoma cell line H4IIE cells (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).
Chromatin Immunoprecipitation Analysis: A representative lot detected enhanced NRF-1 occupancy at the Sod2 promoter region in liver nuclear extracts from mice during S. aureus sepsis (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in Staphylococcus aureus peptidoglycan
(PGN) or dichloromethane (DCM)/carbon monoxide (CO) treated HepG2 cells (MacGarvey, N.C., et al. (2012). Am J Respir Crit Care Med. 185(8):851-861).
Immunocytochemistry Analysis: A representative lot detected NRF-1 in H9C2 rat cardiomyocytes (Carraway, M.S., et al. (2010). Circ Res. 106(11):1722-1730).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-NRF-1 Antibody is validated for use in Western Blotting, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP) for the detection of NRF-1 .
Qualité
Evaluated by Western Blotting in human liver tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected NRF-1 in 10 µg of human liver tissue lysate.
Western Blotting Analysis: 2.0 µg/mL of this antibody detected NRF-1 in 10 µg of human liver tissue lysate.
Description de la cible
~54/58 kDa observed
Forme physique
Format: Purified
Protein A purified
Purified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Activation of mitochondrial biogenesis by heme oxygenase-1-mediated NF-E2-related factor-2 induction rescues mice from lethal Staphylococcus aureus sepsis.
MacGarvey, NC; Suliman, HB; Bartz, RR; Fu, P; Withers, CM; Welty-Wolf, KE; Piantadosi, CA
American Journal of Respiratory and Critical Care Medicine null
Erythropoietin activates mitochondrial biogenesis and couples red cell mass to mitochondrial mass in the heart.
Carraway, MS; Suliman, HB; Jones, WS; Chen, CW; Babiker, A; Piantadosi, CA
Circulation Research null
Peroxisome proliferator-activated receptor ? co-activator 1-? as a critical co-activator of the murine hepatic oxidative stress response and mitochondrial biogenesis in Staphylococcus aureus sepsis.
Cherry, AD; Suliman, HB; Bartz, RR; Piantadosi, CA
The Journal of Biological Chemistry null
Lipopolysaccharide stimulates mitochondrial biogenesis via activation of nuclear respiratory factor-1.
Suliman, HB; Carraway, MS; Welty-Wolf, KE; Whorton, AR; Piantadosi, CA
The Journal of Biological Chemistry null
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