ABE155

Anti-phospho Histone H3.1/H3.2 Antibody (Ser87)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: bovine, mouse, human
• Technique(s): dot blot: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003520
• Numéro d'accès UniProt: P68431
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer87)
• Informations sur le gène: human ... SNCA(6622)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3.1, H3.2 and H3.3 are all closely related Histone H3 variants, that despite differing by only a few amino acids have been shown to have different functional roles.

Spécificité

This antibody recognizes Histone H3.1/H3.2 phosphorylated at Ser87. This antibody demonstrates specificity for phospho Histone H3.1/H3.2 (Ser87), but does not cross react with phospho Histone H3.3, or any other modified and non-modified histones.

Immunogène

Epitope: Phosphorylated at Ser87

KLH-conjugated linear peptide corresponding to human Histone H3.1/H3.2 phosphorylated at Ser87.

Application

Detect Histone H3 using this rabbit polyclonal antibody, Anti-phospho Histone H3.1/H3.2 Antibody (Ser87) validated for use in western blotting & Dot Blot.

Dot Blot (Specificity) Analysis: 1µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in a panel of unmodified and modified Histones.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualité

Evaluated by Western Blotting in Colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3.1/H3.2 (Ser87) in 10 µg of Colcemid treated HeLa acid extract

Description de la cible

~17 kDa observed

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa acid extract

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable