ABC2036

Anti-MLKL

from rabbit

• Synonyme(s) : Mixed lineage kinase domain-like protein, hMLKL
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_689862
• Numéro d'accès UniProt: Q8NB16
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MLKL(197259)

Description

Description générale

Mixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. MLKL contains a protein kinase-like domain; but it lacks several residues required for activity. Its ATP-binding site is located within amino acids 209-217. Two isoforms of MLKL have been described that are produced by alternative splicing. It contains two coiled coil regions (aa 55-84 and 139-180) and coiled coil region 2 is shown to be responsible for its homotrimerization. MLKL is phosphorylated on Thr357/Ser358 by RIP3 kinase that leads to its oligomerization. The oligomeric MLKL then translocates from cytosol to plasma membrane and intracellular membranes where it bind to phosphatidylinositol and cardiolipin and disrupts membrane integrity leading to necrotic cell death. MLKL is reported to play a critical role in TNF-induced necroptosis. (Ref.: Wang, H., et al (2014). Mol. Cell 54(1); 133-146).

Spécificité

This rabbit polyclonal antibody detects Mixed lineage kinase domain-like protein in human cells. It targets an epitope within the first 124 amino acids from the N-terminal region.

Immunogène

Epitope: N-terminus

GST/His-tagged recombinant fragment corresponding to the first 124 amino acids from the N-terminal region of human Mixed lineage kinase domain-like protein (MLKL).

Application

Anti-MLKL, Cat. No. ABC2036, is a rabbit polyclonal antibody that detects Mixed lineage kinase domain-like protein (MLKL) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A 1:500 dilution of from a representative lot detected MLKL in 10 µg of Jurkat cell lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MLKL in human tonsil and human squamous cell carcinoma tissue sections.

Qualité

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MLKL in 10 µg of HT-29 cell lysate.

Description de la cible

~52 kDa observed; 54.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1