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Key Documents

AB2148P

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody

Chemicon®, from rabbit

Synonyme(s) :

TNF-alpha

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

mouse

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
neutralization: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNF(7124)

Spécificité

Reacts with Mouse Tumor Necrosis Factor-alpha (TNF-alpha), a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells.

Immunogène

Highly pure (>98%) recombinant Mouse TNF-alpha.

Application

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody validated for use in ELISA, NEUT & WB.
ELISA: A concentration of at least 0.5μg/mL (100μL/well) of AB2148P is required to detect 0.2-0.4 ng/well of recombinant mTNF-alpha.

Western Blot: A concentration of 0.1-0.2 μg/mL of AB2148P is required to detect 1.5-3.0 ng/lane of mTNF-alpha, under either reducing or non-reducing conditions.

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mTNF-a (0.50 ng/ml), a concentration of 0.010-0.015 μg/ml of AB2148P is required.

Optimal working dilutions must be determined by the end user

Liaison

Replaces: AB1450P

Forme physique

Immunoglobulin purified by affinity chromatography employing immobilized mTNF-alpha matrix. A sterile filtered antibody solution (1mg/mL) was lyophilized from 0.5X PBS, pH 7.2. Reconstitute with sterile distilled water.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Marcos Maciel Junior et al.
Anatomical record (Hoboken, N.J. : 2007), 304(6), 1305-1312 (2020-11-03)
In the present study we investigated the involvement of free fatty acid (FFA) receptors in the anti-inflammatory role of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in dystrophic muscles, by administering FFA blockers in the mdx mouse model of dystrophy.
Evidence for a role of mitogen-activated protein kinase 3/mitogen-activated protein kinase in the development of testicular ischemia-reperfusion injury.
Minutoli, L; Antonuccio, P; Romeo, C; Nicotina, PA; Bitto, A; Arena, S; Polito et al.
Biology of Reproduction null
Ting-Yun Chuang et al.
Oncotarget, 8(30), 49735-49748 (2017-05-26)
The innate immune response is a central process that is activated during pathogenic infection in order to maintain physiological homeostasis. It is well known that dexamethasone (Dex), a synthetic glucocorticoid, is a potent immunosuppressant that inhibits the cytokine production induced
Wan-Ting Tsai et al.
The Journal of biological chemistry, 291(37), 19299-19311 (2016-07-28)
Innate immune responses are important for pathogen elimination and adaptive immune response activation. However, excess inflammation may contribute to immunopathology and disease progression (e.g. inflammation-associated hepatocellular carcinoma). Immune modulation resulting from pattern recognition receptor-induced responses is a potential strategy for
Alba Galan et al.
Investigative ophthalmology & visual science, 58(7), 2852-2862 (2017-06-02)
The p75NTR is a novel therapeutic target validated in a streptozotocin mouse model of diabetic retinopathy. Intravitreal (IVT) injection of small molecule p75NTR antagonist THX-B was therapeutic and resolved the inflammatory, vascular, and neurodegenerative phases of the retinal pathology. To

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