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71772

Millipore

ProteoExtract® Transmembrane Protein Extraction Kit

Synonyme(s) :

Membrane Protein Isolation Kit

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.77

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).

Application

ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins

Caractéristiques et avantages

  • No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
  • Reagents are sufficient to process a total of 40 samples.

Avertissement

Toxicity: Toxic (F)

Autres remarques

Nakamura, E., et al. 2008. Cancer Sci.99, 1390.

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Huimin Luo et al.
Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
Asahi Takeuchi et al.
Nutrients, 12(9) (2020-09-24)
IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk β-lactoglobulin. However, the molecular mechanisms underlying the IIAEK-mediated suppression of intestinal cholesterol absorption are unknown. Therefore, we evaluated the effects of IIAEK on intestinal cholesterol metabolism in a

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