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Millipore

BugBuster® (primary amine-free) Extraction Reagent

Synonyme(s) :

Protein Extraction Reagent

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About This Item

Code UNSPSC :
41106511
Nomenclature NACRES :
NA.77

Forme

liquid

Niveau de qualité

100

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Conditions d'expédition

ambient

Température de stockage

10-30°C

Catégories apparentées

Description générale

BugBuster (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking. The PIPPS buffer used in BugBuster (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Caractéristiques et avantages

Primary Amine-Free Formulation: BugBuster® (primary amine-free) Extraction Reagent is specially designed for applications where primary amines would interfere with protein extract. This means that academic researchers can purify metal-dependent proteins or proteins to be used for immobilization or crosslinking without worrying about the presence of primary amines, which could cause interference.

Enhanced Extraction: The extraction process can be further enhanced by the addition of rLysozyme Solution and Benzonase Nuclease, which helps to efficiently extract and purify proteins from the sample.

Metal Ion Compatibility: The PIPPS buffer used in the primary amine-free formulation of BugBuster® has a similar buffer capacity and pH range as the original Tris-buffered BugBuster® but will not complex metal ions. This makes it an ideal choice for extraction of metal-dependent proteins

Avertissement

Toxicity: Standard Handling (A)

Informations légales

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.

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