69671

Thrombin, Restriction Grade

• Synonyme(s) : Thrombin, Restriction Grade
• Numéro CAS: 9002-04-4
• Code UNSPSC : 12352202

Propriétés

• Source biologique: human plasma
• Niveau de qualité: 400
• Forme: liquid
• Fabricant/nom de marque: Novagen^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): protein extraction: suitable
• Adéquation: suitable for additive or modifier in the separation of proteins or peptides
• Application(s): life science and biopharma
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Composants

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Définition de l'unité

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Notes préparatoires

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1