681660-M

N-WASP-Inhibitor, 187-1

The N-WASP-Inhibitor, 187-1 controls the biological activity of neural Wiskott Aldrich syndrome protein (N-WASP). This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonyme(s) : N-WASP-Inhibitor, 187-1, cyclo( LK DF DP DF LF DP LQ)₂
• Formule empirique (notation de Hill): C₉₆H₁₂₂N₁₈O₁₆
• Poids moléculaire : 1784.11
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Couleur: white
• Solubilité: DMSO: 1 mg/mL; acetic acid: 5%
• Température de stockage: −20°C

Description

Description générale

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~ 2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and prevent the activation of actin-related protein 2/3 (Arp2/3) complex by inhibiting the interaction between N-WASP and Arp2/3.

A 14-amino acid cyclic peptide that potently blocks phosphatidylinositol 4,5-bisphosphate (PIP₂)-induced actin assembly in Xenopus egg cytoplasmic extract (IC₅₀ ~2 µM). Shown to directly bind to neural Wiskott Aldrich syndrome protein (N-WASP) with high-affinity, and allosterically stabilize the autoinhibited conformation of N-WASP. It prevents the activation of actin-related protein 2/3 (Arp2/3) complex by blocking the interaction between N-WASP and Arp2/3.

Actions biochimiques/physiologiques

Primary Target
Neural Wiskott Aldrich syndrome protein (N-WASP)

Target IC₅₀: ~ 2 µM blocking phosphatidylinositol 4,5-bisphosphate (PIP2)-induced actin assembly in Xenopus egg cytoplasmic extract

Avertissement

Toxicity: Standard Handling (A)

Séquence

cyclo(LLys-DPhe-DPro-DPhe-LPhe-DPro-LGln)₂

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Autres remarques

Peterson, J.R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 10624.
Suetsugu, S., et al. 2001. J. Biol. Chem.276, 33175.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable