650366-M

Tryptase, Human Lung

• Numéro de classification (Commission des enzymes): 3.4.21.59 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Activité spécifique: ≥50 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Température de stockage: −20°C

Description

Description générale

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Reported to upregulate neoangiogenesis in carcinogenesis of the uterine cortex. Tryptase is also an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Tryptase is an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Avertissement

Toxicity: Irritant (B)

Définition de l'unité

One unit is defined as the amount of enzyme that gives a change of 1 absorbance per min at 410 nm using N-benzoyl-Phe-Val-Arg-pNA as a substrate in 30 min at 25°C.

Forme physique

In 1 M NaCl, 50 mM NaOAc, 50 µM heparin, pH 5.0.

Notes préparatoires

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Benitez-Bribiesca, L., et al. 2001. J. Histochem. Cytochem.49, 1061.
He, S., et al. 1998. J. Pharmacol. Exp. Ther. 286, 289.
Molino, M., et al. 1997. J. Biol. Chem. 272, 4043.
Schwartz, L.B., and Bradford, T.R. 1986. J. Biol. Chem. 261, 7372.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1