648310-M

Tris(hydroxyméthyl)aminométhane

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

• Synonyme(s) : 2-Amino-2-(hydroxyméthyl)propane-1,3-diol, Base Tris, THAM, Trométamol
• Formule linéaire : NH₂C(CH₂OH)₃
• Numéro CAS: 77-86-1
• Poids moléculaire : 121.14
• Numéro Beilstein : 741883
• Numéro MDL: MFCD00004679
• Code UNSPSC : 12161705

Propriétés

• product name: Tris(hydroxyméthyl)aminométhane, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
• Qualité: Molecular Biology
• Niveau de qualité: 100
• Pureté: ≥99% (perchloric acid titration)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white
• Plage de pH utile: 7-9
• pKa (25 °C): 8.1
• Point d'ébullition: 219-220 °C/10 mmHg (lit.)
• Pf: 167-172 °C (lit.)
• Solubilité: water: 500 mg/mL
• Traces de cations: heavy metals: ≤0.0005%
• Absorption: ≤0.02 at 280 nm; ≤0.03 at 260 nm
• Activité étrangère: RNases, DNases, proteases, none detected
• Température de stockage: 10-30°C
• Chaîne SMILES: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

Description générale

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Application

• A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).


• Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Avertissement

Toxicity: Irritant (B)

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable