567333

SNAPtide Botulinum Toxin A Substrate, Fluorogenic

≥90% (HPLC), lyophilized, FRET substrate, Calbiochem^®

• Synonyme(s) : Botulinum Toxin Assay
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: SNAPtide Botulinum Toxin A Substrate, Fluorogenic, SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).
• Niveau de qualité: 100
• Pureté: ≥90% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Fluorescence: λ_ex ~326 nm; λ_em ~423 nm
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Substrate for the native botulinum toxin

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from water.

Notes préparatoires

Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.

Reconstitution

Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable