525200-M

Phospholipase D, Streptomyces chromofuscus

• Synonyme(s) : Phospholipase D, Streptomyces chromofuscus, PLD
• Numéro CAS: 9001-87-0
• Numéro de classification (Commission des enzymes): 3.1.4.4 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131897
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized solid
• Niveau de qualité: 100
• Activité spécifique: ≥50 units/mg dry wt.
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated
• Couleur: brownish
• Solubilité: 10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON^™ X-100: 1 mg/mL
• Activité étrangère: Aatalase, glucose oxidase, none detected
• Température de stockage: −20°C

Description

Description générale

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.

Native phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.

Autres remarques

Kurz, T., et al. 1993. Circ. Res. 72, 701.
Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
Yamamoto, I., et al. 1993. Biochim. Biophys. Acta1145, 293.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P304 + P340 + P312 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3