524627
Phosphatase Inhibitor Cocktail Set III
Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.
Synonyme(s) :
Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77
Forme
liquid
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Conditions d'expédition
wet ice
Température de stockage
−20°C
Catégories apparentées
Description générale
Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.
Application
Phosphatase Inhibitor Cocktail Set III has been used:
- as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
- to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
- as a component in cell lysis buffer to extract total proteins for western blotting
Actions biochimiques/physiologiques
Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no
Caractéristiques et avantages
- Ready-to-use formulations for convenient use
- High-quality products for consistent and reproducible performance,
- Flexible options with a range of cocktails targeting different categories of phosphatases
Composants
Each vial contains 1 ml of aqueous solution with the following components:
- 50 mM Sodium Fluoride
- 10 mM ß-Glycerophosphate (Cat. No. 35675)
- 10 mM Sodium Pyrophosphate Decahydrate
- 1 mM Sodium Orthovanadate
Avertissement
Toxicity: Toxic (F)
Forme physique
In H₂O.
Reconstitution
For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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