475989

MTT

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan.

• Synonyme(s) : MTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide, Thiazole Blue
• Formule empirique (notation de Hill): C₁₈H₁₆BrN₅S
• Numéro CAS: 298-93-1
• Poids moléculaire : 414.32
• Numéro MDL: MFCD00011964
• Code UNSPSC : 12171500
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (silver nitrate titration)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤0.5% Sulfated ash
• Couleur: yellow
• Solubilité: PBS: 2 mg/mL
• Traces de cations: heavy metals: ≤0.001%
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• Clé InChI: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Description

Description générale

Membrane-permeable yellow dye that is reduced by mitochondrial reductases in living cells to form the dark blue product, MTT-formazan. Spectrophotometric measurement of MTT-formazan at 540 or 570 nm allows quantitation of cell viability. Used as a direct indicator of cytotoxicity (such as for screening cancer drugs) and apoptosis. An electron acceptor used for studying NADP-dependent dehydrogenases.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
Used as a direct indicator of cytotoxicity and apoptosis.

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C.). Stock solutions are stable for up to 1 month at -20°C.

Autres remarques

Liu, Y., et al. 1997. J. Neurochem.69, 581.
Abdallah, M.A. 1980. Eur. J. Biochem. 112, 331.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335,H341
• Conseils de prudence: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable