420291

IPTG, d'origine non animale, de grande pureté

Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.

• Synonyme(s) : IPTG, d'origine non animale, de grande pureté, Isopropyl-β-D-thiogalactopyranoside
• Formule empirique (notation de Hill): C₉H₁₈O₅S
• Numéro CAS: 367-93-1
• Poids moléculaire : 238.30
• Numéro MDL: MFCD00063273
• Code UNSPSC : 12352201
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Impuretés: Dioxane, none detected
• Couleur: white to off-white
• Solubilité: water: 100 mg/mL; methanol: soluble
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• Clé InChI: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

Description générale

Inducteur de β-D-galactosidase. Composé utilisé pour la stimulation de la β-galactosidase dans les systèmes cellulaires dans lesquels le dioxane perturberait le fonctionnement cellulaire normal. Ne contient aucun produit d′origine animale.

Application

• Mobile-CRISPRi as a powerful tool for modulating Vibrio gene expression.: This article explores the use of Mobile-CRISPRi for targeted gene regulation in Vibrio species, highlighting potential applications in microbial genetics and biotechnological innovations, including the manipulation of gene expression pathways facilitated by IPTG-induced systems (Geyman et al., 2024).


• A new Zymomonas mobilis platform strain for the efficient production of chemicals.: This research describes the development of a Zymomonas mobilis strain engineered for enhanced chemical production, utilizing IPTG for controlled expression of metabolic pathways, showcasing significant implications for biofuel production and industrial biotechnology (Frohwitter et al., 2024).


• Biophysical characterization and insights into the oligomeric nature of CD2-associated protein.: The study provides a detailed biophysical analysis of CD2-associated protein, employing IPTG-induced expression to elucidate its oligomeric structure and function in cellular signaling, offering insights into therapeutic target development (Qadri et al., 2024).


• Effective delivery of anti-PD-L1 siRNA with human heavy chain ferritin (HFn) in acute myeloid leukemia cell lines.: This research utilizes IPTG-inducible systems to study the delivery of therapeutic siRNAs in leukemia treatment, demonstrating the potential of IPTG in enhancing the efficacy of gene therapy approaches in oncology (Rajabinejad et al., 2024).


• Heterologous expression of nattokinase in E. coli: Biochemical characterization and functional analysis of fibrin binding residues.: This article details the heterologous expression of nattokinase in E. coli, facilitated by IPTG induction, focusing on its potential applications in thrombolytic therapy and its biochemical properties relevant to medical biochemistry (Jain et al., 2024).

Avertissement

Toxicité : manipulation standard (A)

Reconstitution

Après reconstitution, diviser en aliquotes et congeler (à -20 °C). Les solutions mères sont stables jusqu′à 6 mois à -20 °C.

Autres remarques

Lama, J., et al. 1992. Gene117, 185.
Zhang, Y., and Moss, B. 1991. Natl. Acad. Sci. USA88, 1511.
Rodriquez, J.F., and Smith, G.L. 1990. Nucleic Acids Res.18, 5347.
Date, T., et al. 1988. Biochem. Biophys. Res. Commun.151, 598.
Lee, S.G., et al. 1988. Biochemistry27, 2983.
Cho, S., et al. 1985. Biochem. Biophys. Res. Commun.128, 1268.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable