391338

HEPES

≥99% dry basis (titration), crystalline powder, amniosulfonic acid buffer

• Synonyme(s) : Acide 4-(2-hydroxyéthyl)pipérazine-1-éthanesulfonique, Acide N-(2-hydroxyéthyl)pipérazine-N′-(2-éthanesulfonique)
• Formule empirique (notation de Hill): C₈H₁₈N₂O₄S
• Numéro CAS: 7365-45-9
• Poids moléculaire : 238.30
• Numéro Beilstein : 883043
• Numéro MDL: MFCD00006158
• Code UNSPSC : 12352106

Propriétés

• product name: HEPES, ULTROL^® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer
• Niveau de qualité: 100
• Pureté: ≥99% dry basis (titration)
• Forme: crystalline powder
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Couleur: white
• Plage de pH utile: 6.8-8.2
• pKa (25 °C): 7.5
• Solubilité: water: 1 M
• Traces d'anions: chloride (Cl^-): ≤0.01%; sulfate (SO₄^2-): ≤0.05%
• Traces de cations: Na: ≤0.1%; heavy metals: ≤1 ppm
• Conditions d'expédition: ambient
• Température de stockage: 15-25°C
• Chaîne SMILES: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• Clé InChI: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Description générale

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pK_a of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pK_a of 7.48 at 25°C.

Absorbance (1.0 M, H₂O, 260 nm):≤0.05.

Absorbance (1.0 M, H₂O, 280 nm): ≤0.04

Avertissement

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable