341583

FGF-2, Basic, Bovine

• Synonyme(s) : FGF-2, Basic, Bovine, nbbFGF, Fibroblast Growth Factor, Basic, Bovine
• Numéro CAS: 106096-93-9
• Numéro MDL: MFCD00081382
• Code UNSPSC : 12352202

Propriétés

• Pureté: ≥97% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: <0.1 ng/μg Endotoxin (ng/μg FGF)
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. A multifunctional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Known to activate phopholipase D in endothelial cells. Higher concentrations are found in the brain and pituitary gland. Purified from bovine brain by sequential chromatography.

Native, bovine basic fibroblast growth factor. A multi-functional factor with chemotactic activity for numerous cell types. Mitogenic to neuroectoderm- and mesoderm-derived cells. Plays a role in the proliferation of neuronal precursors and in neuronal differentiation. A potent angiogenic factor both in vivo and in vitro. Activates phospholipase D in endothelial cells.

Actions biochimiques/physiologiques

ED₅₀ = 50-150 pg/ml, in the absence of heparin, for stimulation of ³H-thymidine incorporation by NR6R-3T3 fibroblasts

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from sterile-filtered 20 mM Tris-HCl, 1M NaCl, 50 µg BSA/µg FGF, pH 7.0.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.

Autres remarques

Ohmiya, M., et al. 2001. J. Neurosci. Res.65, 228.
Murai, N., et al. 1996. J. Neurosurg. 85, 1072.
Thomas, K. 1987. Methods Enzymol.147, 120.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3