325885

c-MET, GST-Fusion, Human, Recombinant, S. frugiperda

c-MET, GST-Fusion, Human, Recombinant (aa 956-1390) fused at the N-terminus to a GST-His6-thrombin cleavage site and expressed in S. frugiperda. c-Met is a receptor tyrosine kinase activated by HGF.

• Synonyme(s) : Hepatocyte growth factor receptor tyrosine kinase
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Produit recombinant: expressed in baculovirus infected Sf21 cells
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥30 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Recombinant, human c-MET (amino acids 956-1390) (Genebank entry NM_000245) fused at the N-terminus to a GST-His₆-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. c-Met is a receptor tyrosine kinase activated by hepatocyte growth factor (HGF)/scatter factor (SF). c-MET is a plasminogen-like protein thought to be a humoral mediator of liver regeneration.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala, Glu, Lys, Tyr)_6:2:5:1 per min at 30°C, using variable concentrations of ATP (0.1-1.48 µM).

Forme physique

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Palka, H.L., et al. 2003. J. Biol. Chem.278, 5278.
Vadnais, J., et al. 2002. J. Biol. Chem.277, 48342.
Crostella, L., et al. 2001. Oncogene20, 3735.
Guiton, M., et al. 1994. J. Biol. Chem269, 30370.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1